Difference between revisions of "Part:BBa K1149020"
Margarita K (Talk | contribs) |
Margarita K (Talk | contribs) |
||
| Line 8: | Line 8: | ||
We were inspired by the beautiful blue coral colour and taught the bacteria how to write our name with it. | We were inspired by the beautiful blue coral colour and taught the bacteria how to write our name with it. | ||
| − | + | https://static.igem.org/mediawiki/igem.org/f/fb/PlasticityBluePlate.JPG | |
| − | We have observed change in colour of an Overnight culture with the construct. However, it was not as blue as the | + | We have observed change in colour of an Overnight culture with the construct. However, it was not as blue as the |
https://static.igem.org/mediawiki/igem.org/f/f5/AmilCP_cultureICL.jpg | https://static.igem.org/mediawiki/igem.org/f/f5/AmilCP_cultureICL.jpg | ||
Revision as of 10:21, 8 September 2013
Promoter J23104 - RBS B0034 - amilCP
The chromoprotein from the coral Acropora millepora, amilCP, naturally exhibits strong color when expressed. The protein has an absorbance maximum at 588 nm giving it a blue/purple color visible to the naked eye, thereby requiring no instruments to observe.
The blue coloration made it very easy to select the ligations with the insert from the transformation plate:
We were inspired by the beautiful blue coral colour and taught the bacteria how to write our name with it.
We have observed change in colour of an Overnight culture with the construct. However, it was not as blue as the
We have verified the sequence and detected 3 mutations compared to the expected results. However, these are located after the prefix and at the scar site and do not affect the coding region or the function of the construct.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]