Difference between revisions of "Part:BBa K1114005:Design"

(→‎Design Notes)
(→‎Design Notes)
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===Design Notes===
 
===Design Notes===
 
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<html>
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This is the <a href="http://2013.igem.org/Team:BostonU/MoCloChara">MoClo</a> formatted part of BBa_R0079 with EB fusion sites. See the <a href="https://parts.igem.org/Part:BBa_R0079">BBa_R0079</a> page for full information on the part.  
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This is the <a href="http://2013.igem.org/Team:BostonU/MoCloChara">MoClo</a> formatted part of BBa_J23104 with EB fusion sites. See the <a href="https://parts.igem.org/Part:BBa_R0079">BBa_J23104</a> page for full information on the part.  
 
</html>
 
</html>
  
 
Summary of modifications from original part:
 
Summary of modifications from original part:
 
Backbone has one added SpeI site in front of gene, BsaI sites, and 4bp fusion sites.
 
Backbone has one added SpeI site in front of gene, BsaI sites, and 4bp fusion sites.
 +
 +
The fusion site letters refer to 4bp fusion sites: A=GGAG; B=TACT; C=AATG; D=AGGT; E=GCTT; F = CGCT; G=TGCC; H=ACTA.
  
 
===Source===
 
===Source===

Revision as of 17:19, 7 September 2013


The MoClo format of BBa_J23104 with AB fusion sites.


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 11
    Illegal NheI site found at 34
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This is the MoClo formatted part of BBa_J23104 with EB fusion sites. See the BBa_J23104 page for full information on the part.

Summary of modifications from original part: Backbone has one added SpeI site in front of gene, BsaI sites, and 4bp fusion sites.

The fusion site letters refer to 4bp fusion sites: A=GGAG; B=TACT; C=AATG; D=AGGT; E=GCTT; F = CGCT; G=TGCC; H=ACTA.

Source

iGEM Distribution Kit

References