Difference between revisions of "Part:BBa K1216000"
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<partinfo>BBa_K1216000 short</partinfo> | <partinfo>BBa_K1216000 short</partinfo> | ||
| − | ''gusA'' (also called uidA[[Part:BBa_K1216000#References|<sup>[1]</sup>]]) encodes β-Glucuronidase, an intracellular enzyme that catalyzes the hydrolysis of β-D-glucuronides. [[File: | + | ''gusA'' (also called uidA[[Part:BBa_K1216000#References|<sup>[1]</sup>]]) encodes β-Glucuronidase, an intracellular enzyme that catalyzes the hydrolysis of β-D-glucuronides. [[File:Glucuronidase_3d_model.jpg|thumb| 3D representation of the alkaline phosphatase from [http://www.rcsb.org/pdb/explore/explore.do?structureId=3K46 RCSB] ]] |
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Generally it can be used as reporter enzyme with detection by biochemical activity assays, immunological assays or by histochemical staining of tissue sections or cells[[Part:BBa_K1216000#References|<sup>[3]</sup>]]. | Generally it can be used as reporter enzyme with detection by biochemical activity assays, immunological assays or by histochemical staining of tissue sections or cells[[Part:BBa_K1216000#References|<sup>[3]</sup>]]. | ||
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<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> | ||
<partinfo>BBa_K1216000 SequenceAndFeatures</partinfo> | <partinfo>BBa_K1216000 SequenceAndFeatures</partinfo> | ||
Revision as of 08:45, 6 September 2013
β-Glucuronidase (gusA) from Bacillis Subtilis
gusA (also called uidA[1]) encodes β-Glucuronidase, an intracellular enzyme that catalyzes the hydrolysis of β-D-glucuronides.
A form of this protein with added TEV and poly-HIS tags can be found here.
Usage and Biology
β-Glucuronidase is used as a fusion protein marker in higher plants, due to them lacking intrinsic β-Glucuronidase activity[2]. Generally it can be used as reporter enzyme with detection by biochemical activity assays, immunological assays or by histochemical staining of tissue sections or cells[3].
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 538
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
References
- [http://ecoliwiki.net/colipedia/index.php/uidA:Gene ecoliwiki]
- Jefferson A R, "GUS fusions: beta-glucuronidase as a sensitive and versatile gene fusion marker in higher plants.", EMBO J. 1987 December 20; 6(13): 3901–3907 [http://www.ncbi.nlm.nih.gov/pmc/articles/PMC553867/]
- [http://www.sigmaaldrich.com/etc/medialib/docs/Sigma/Bulletin/gusabul.Par.0001.File.tmp/gusabul.pdf Sigma Aldrich]