Difference between revisions of "Part:BBa K1155000:Design"

 
(→‎Design Notes)
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===Design Notes===
 
===Design Notes===
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This promoter region correspond to a modified ndh promoter region of Escherichia coli. The second FNR binding site (FNRII) was modified to obtain a perfect consensus motif, improving the strength of the repression (Meng et al., 1997).  
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This promoter region corresponds to a modified ndh promoter region of Escherichia coli. The second FNR binding site (FNRII) was modified to obtain a perfect consensus motif, improving the strength of the repression (Meng et al., 1997).
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+
  
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'''Reference'''
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Meng W., Green J. and Guest J. R. (1997). FNR-dependent repression of ndh gene expression requires two upstream FNR-binding sites, Microbiology, '''143''', 1521-1532.
  
 
===Source===
 
===Source===

Revision as of 14:13, 5 September 2013


Pndh*


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This promoter region corresponds to a modified ndh promoter region of Escherichia coli. The second FNR binding site (FNRII) was modified to obtain a perfect consensus motif, improving the strength of the repression (Meng et al., 1997).

Reference Meng W., Green J. and Guest J. R. (1997). FNR-dependent repression of ndh gene expression requires two upstream FNR-binding sites, Microbiology, 143, 1521-1532.

Source

Escherichia coli K12 genome sequence

References