Difference between revisions of "Part:BBa K1184000:Design"
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===References=== | ===References=== | ||
− | <p><sup>1 | + | <p><sup>1</sup> Bulina, Maria E, Chudakov, Dmitriy M, Britanova, Olga V, Yanushevich, Yurii G, Staroverov, Dmitry B, Chepurnykh, Tatyana V, Merzlyak, Ekaterina M, Shkrob, Maria A, Lukyanov, Sergey, Lukyanov and Konstantin A. <br /> |
− | + | ||
− | + | ||
− | + | ||
− | + | ||
<i>A Genetically Encoded Photosensitizer</i><br /> | <i>A Genetically Encoded Photosensitizer</i><br /> | ||
Nature Biotechnology 2006 24: 95-99. http://dx.doi.org/10.1038/nbt1175 | Nature Biotechnology 2006 24: 95-99. http://dx.doi.org/10.1038/nbt1175 | ||
+ | </p> | ||
+ | <p><sup>2</sup> Sergei Pletnev, Nadya G. Gurskaya, Nadya V. Pletneva, Konstantin A. Lukyanov, Dmitri M. Chudakov, Vladimir I. Martynov, Vladimir O. Popov, Mikhail V. Kovalchuk, Alexander Wlodawer, Zbigniew Dauter, and Vladimir Pletnev.<br /> | ||
+ | <i>Structural Basis for Phototoxicity of the Genetically Encoded Photosensitizer KillerRed</i><br /> | ||
+ | Journal of Biological Chemistry 2009 284: 32028-32039. First Published on September 8, 2009, doi:10.1074/jbc.M109.054973 | ||
</p> | </p> |
Revision as of 20:37, 4 September 2013
KillerRed
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 151
Illegal BsaI.rc site found at 442
Design Notes
This sequence is not codon optimized for E. coli. Sequence contains 14 rare proline codons (CCC) and a rare arginine codon (AGA).
KillerRed has catalytic residues E70 and S121. The chromophore forms from the backbone of Q67, Y68 and G69.1,2
Source
Engineered from anm2CP. Provided by the Bruchez lab at Carnegie Mellon
References
1 Bulina, Maria E, Chudakov, Dmitriy M, Britanova, Olga V, Yanushevich, Yurii G, Staroverov, Dmitry B, Chepurnykh, Tatyana V, Merzlyak, Ekaterina M, Shkrob, Maria A, Lukyanov, Sergey, Lukyanov and Konstantin A.
A Genetically Encoded Photosensitizer
Nature Biotechnology 2006 24: 95-99. http://dx.doi.org/10.1038/nbt1175
2 Sergei Pletnev, Nadya G. Gurskaya, Nadya V. Pletneva, Konstantin A. Lukyanov, Dmitri M. Chudakov, Vladimir I. Martynov, Vladimir O. Popov, Mikhail V. Kovalchuk, Alexander Wlodawer, Zbigniew Dauter, and Vladimir Pletnev.
Structural Basis for Phototoxicity of the Genetically Encoded Photosensitizer KillerRed
Journal of Biological Chemistry 2009 284: 32028-32039. First Published on September 8, 2009, doi:10.1074/jbc.M109.054973