Difference between revisions of "Part:BBa K1129002"

 
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<partinfo>BBa_K1129002 short</partinfo>
 
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<partinfo>BBa_K1129002 parameters</partinfo>
 
<partinfo>BBa_K1129002 parameters</partinfo>
 
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===DATA===
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<p align=center>https://static.igem.org/mediawiki/2013/a/ac/Ech900.jpg</p>
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'''Figure 1''' Compound generation identification by GC-MS. Chromatograms(left) and mass spectra(Right) for select peaks are shown. Structures represent predictions based on library matching or comparison to standards. Controls represent plasmids missing the gene of interest. A) Internal control using vanillin(10.486). B) Conversion of Ferulic acid to vanillin acid by constitutive expressed Fcs and Ech through the Feuroyl CoA intermediate, not shown. C) Conversion of Ferulic acid to vanillin acid by Arabinose inducible expressed Fcs and Ech through the Feuroyl CoA intermediate, not shown.

Revision as of 02:47, 25 October 2013

Enoyl-CoA hydratase/aldolase (ECH) from Pseudomonas putida

This is a enoyl-CoA hydratase/aldolase from Pseudomonas putida KT2440 which converts feruloyl-CoA to vanillin

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 755
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 184
    Illegal NgoMIV site found at 202
    Illegal NgoMIV site found at 241
    Illegal AgeI site found at 178
  • 1000
    COMPATIBLE WITH RFC[1000]


DATA

Ech900.jpg


Figure 1 Compound generation identification by GC-MS. Chromatograms(left) and mass spectra(Right) for select peaks are shown. Structures represent predictions based on library matching or comparison to standards. Controls represent plasmids missing the gene of interest. A) Internal control using vanillin(10.486). B) Conversion of Ferulic acid to vanillin acid by constitutive expressed Fcs and Ech through the Feuroyl CoA intermediate, not shown. C) Conversion of Ferulic acid to vanillin acid by Arabinose inducible expressed Fcs and Ech through the Feuroyl CoA intermediate, not shown.