Difference between revisions of "Part:BBa K1216002"
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<partinfo>BBa_K1216002 short</partinfo>
 
<partinfo>BBa_K1216002 short</partinfo>
  
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Acetyl esterase-This is a cytosolic hydrolase that can catalyse esters of p-nitrophebyl derivatives  
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Acetyl esterase - This is a cytosolic hydrolase that can catalyze hydrlysis of esters of p-nitrophenyl derivatives  
  
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A form of this protein with added TEV and poly-HIS tags can be found [[Part:BBa_K1216006| here]].
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===Usage and Biology===
 
===Usage and Biology===
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The acetyle esterase, apart from its hydrolase activity also binds competitively to malT, a transcription activator for the maltose operon, thus inhibiting regulation of genes required for maltose catabolism[[Part:BBa_K1216002#References|<sup>[1]</sup>]] [[Part:BBa_K1216002#References|<sup>[2]</sup>]].
  
 
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<partinfo>BBa_K1216002 parameters</partinfo>
 
<partinfo>BBa_K1216002 parameters</partinfo>
 
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===References===
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# [http://www.ecocyc.org/new-image?type=GENE&object=EG11101 Ecocyc]
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# [http://www.ecocyc.org/ECOLI/NEW-IMAGE?type=ENZYME&object=MONOMER0-158&orgids=ECOLI Ecocyc]

Revision as of 09:17, 6 September 2013

Acetyl esterase (aes) from Escherichia Coli

Acetyl esterase - This is a cytosolic hydrolase that can catalyze hydrlysis of esters of p-nitrophenyl derivatives

A form of this protein with added TEV and poly-HIS tags can be found here.

Usage and Biology

The acetyle esterase, apart from its hydrolase activity also binds competitively to malT, a transcription activator for the maltose operon, thus inhibiting regulation of genes required for maltose catabolism[1] [2].

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 27
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 951


References

  1. [http://www.ecocyc.org/new-image?type=GENE&object=EG11101 Ecocyc]
  2. [http://www.ecocyc.org/ECOLI/NEW-IMAGE?type=ENZYME&object=MONOMER0-158&orgids=ECOLI Ecocyc]