Difference between revisions of "Part:BBa K1195003:Design"

 
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===Design Notes===
 
===Design Notes===
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The hard terminator was added.  
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The promoter Qrr4 from Cholera that is sensitive to the cascade of proteins that are activated when cholera auto-inducers are present was attached onto RFP using soeing PCR. A hard terminator was added to the end of RFP.
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===Source===
 
===Source===

Revision as of 23:19, 16 September 2013


Promoter Qrr4 + RFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 741
    Illegal AgeI site found at 853
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The promoter Qrr4 from Cholera that is sensitive to the cascade of proteins that are activated when cholera auto-inducers are present was attached onto RFP using soeing PCR. A hard terminator was added to the end of RFP.

Source

Qrr4 is from Vibrio Cholerae. RFP is from the plasmid used to send parts to iGEM.

References