Difference between revisions of "Part:BBa K1041000:Design"
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===Design Notes=== | ===Design Notes=== | ||
− | + | Team NRP-UEA_Norwich 2013 added an NdeI restriction site at the start of the RFP coding region of [[BBa_J04450]] using mutagenesis. This was to allow either the promoter region or RFP gene to be excised and exchanged for a different promoter or gene. We could then use this biobrick to create new parts like [[Bba_K1041002]]. | |
===Source=== | ===Source=== |
Revision as of 16:12, 13 September 2013
RFP Coding Device
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 781
Illegal AgeI site found at 893 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
Team NRP-UEA_Norwich 2013 added an NdeI restriction site at the start of the RFP coding region of BBa_J04450 using mutagenesis. This was to allow either the promoter region or RFP gene to be excised and exchanged for a different promoter or gene. We could then use this biobrick to create new parts like Bba_K1041002.