Difference between revisions of "Part:BBa K1188001"

Revision as of 23:40, 22 September 2013

Freiburg amilCP Color Tag

We have put the amilCP blue chromoprotein into Freiburg format so that it can be used to create fusion proteins. By putting AmilCP into Freiburg format this allows AmilCP to be fused with another protein so that other proteins can be color tagged making identification and tracking much easier to do.

You may have to try attaching this protein in front or behind your protein depending on where the active site is. If it turns out to cause problems on either side a linked protein can be used. We have a Freiburg linked available as part Part:BBa_K1188006.

AmilCP absorbs at 588 nm.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 4
Illegal AgeI site found at 670
1000
COMPATIBLE WITH RFC[1000]

@@ Line 1: / Line 1: @@
 __NOTOC__
 <partinfo>BBa_K1188001 short</partinfo>
-We have put the amilCP blue chromoprotein into Freiburg format so that it can be used to create fusion proteins.  This is useful for gel purifying proteins, as documented on the 2013 CU-Boulder iGEM Wiki.
+We have put the amilCP blue chromoprotein into Freiburg format so that it can be used to create fusion proteins.  By putting AmilCP into Freiburg format this allows AmilCP to be fused with another protein so that other proteins can be color tagged making identification and tracking much easier to do.
+You may have to try attaching this protein in front or behind your protein depending on where the active site is. If it turns out to cause problems on either side a linked protein can be used. We have a Freiburg linked available as part [[Part:BBa_K1188006]].
+AmilCP absorbs at 588 nm.
+<!-- Add more about the biology of this part here
+===Usage and Biology===
+<!-- -->
+[[File:Cu_Amilcp.png|200px]]
 <!-- Add more about the biology of this part here