Difference between revisions of "Part:BBa K1088006"

 
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__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K1088006 short</partinfo>
 
<partinfo>BBa_K1088006 short</partinfo>
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A strong constitutive promoter: BBa_J23100
 
A strong constitutive promoter: BBa_J23100
 
A strong RBS: BBa_J15001
 
A strong RBS: BBa_J15001
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The coding region of Dxs from B. sub: BBa_K1088000
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The coding region of ''dxs'' from ''B. subtilis'': BBa_K1088000
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A 10 aa Protein Linker: BBa_K105012
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A 10 amino acid protein linker: BBa_K105012
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And the AmilCP chromoprotein: BBa_K592009
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The AmilCP chromoprotein: BBa_K592009
  
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The idea was to detect expression of dxs protein by the reporter amilCP fused via a linker to the Dxs protein. Unfortunately it seems as though the amilCP protein does not work well as a protein-fusion reporter, and the color change was vague.  
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The idea was to detect expression of the Dxs protein by the reporter amilCP fused via a linker to the Dxs protein. Unfortunately it seems that the amilCP protein does not work well as a protein-fusion reporter, and the color change was vague.  
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 12:37, 26 October 2013

B. subtilis dxs-AmilCP fusion (constitutive promoter)

This composite part consists of 5 sub parts

A strong constitutive promoter: BBa_J23100 A strong RBS: BBa_J15001 The coding region of dxs from B. subtilis: BBa_K1088000 A 10 amino acid protein linker: BBa_K105012 The AmilCP chromoprotein: BBa_K592009

The idea was to detect expression of the Dxs protein by the reporter amilCP fused via a linker to the Dxs protein. Unfortunately it seems that the amilCP protein does not work well as a protein-fusion reporter, and the color change was vague.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1108
    Illegal EcoRI site found at 1765
    Illegal PstI site found at 1166
    Illegal PstI site found at 1612
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1108
    Illegal EcoRI site found at 1765
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal PstI site found at 1166
    Illegal PstI site found at 1612
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1108
    Illegal EcoRI site found at 1765
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1108
    Illegal EcoRI site found at 1765
    Illegal PstI site found at 1166
    Illegal PstI site found at 1612
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1108
    Illegal EcoRI site found at 1765
    Illegal PstI site found at 1166
    Illegal PstI site found at 1612
    Illegal NgoMIV site found at 1065
    Illegal AgeI site found at 958
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 907
    Illegal SapI.rc site found at 1606