Difference between revisions of "Part:BBa K1065104:Design"
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===Design Notes=== | ===Design Notes=== | ||
The gene sequence was extracted using the following primers: | The gene sequence was extracted using the following primers: | ||
| + | |||
>Primer Forward: prefix + RBS + spacer + ATG... | >Primer Forward: prefix + RBS + spacer + ATG... | ||
| + | |||
GAATTCGCGGCCGCTTCTAGAGAAGGAGGAACTACTATGGCAAAACACCTTTTT | GAATTCGCGGCCGCTTCTAGAGAAGGAGGAACTACTATGGCAAAACACCTTTTT | ||
>Primer Reverse: sequence_TAATAA_suffix | >Primer Reverse: sequence_TAATAA_suffix | ||
| − | |||
| − | |||
| + | GAATTCGCGGCCGCTTCTAGAGAAGGAGGAACTACTATGGCAAAACACCTTTTT | ||
===Source=== | ===Source=== | ||
Revision as of 13:47, 14 August 2013
RBS + SAM synthetase
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 116
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 696
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The gene sequence was extracted using the following primers:
>Primer Forward: prefix + RBS + spacer + ATG...
GAATTCGCGGCCGCTTCTAGAGAAGGAGGAACTACTATGGCAAAACACCTTTTT
>Primer Reverse: sequence_TAATAA_suffix
GAATTCGCGGCCGCTTCTAGAGAAGGAGGAACTACTATGGCAAAACACCTTTTT
Source
SAM synthetase gene sequence extracted by PCR from E. coli MG1655 strain genome.