Difference between revisions of "Part:BBa K1210000"

Revision as of 15:35, 20 September 2013

LacI-CFP-PK401-YFP

Reporter construct for measuring the frameshifting efficiency of the PK401 pseudoknot. Produces a reporter of framshift-induction. If translation continues in the 0 frame, a stop codon will be reached to give CFP as the final product. If frameshifting occurs due to the PK401 pseudoknot, translation will continue in the -1 frame to produce a CFP-YFP fusion protein with which FRET can be measured.

This construct was designed to verify the applicability of frameshift inducing elements in the programming of protein expression ratios.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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 __NOTOC__
 <partinfo>BBa_K1210000 short</partinfo>
-Reporter construct for measuring the frameshifting efficiency of the PK401 pseudoknot. Produces a reporter of framshift-induction. If YFP coding region is read in the 0 frame, FRET can be observed. If the YFP coding region is translated in the -1 frame, there will be no FRET.
+Reporter construct for measuring the frameshifting efficiency of the PK401 pseudoknot. Produces a reporter of framshift-induction. If translation continues in the 0 frame, a stop codon will be reached to give CFP as the final product. If frameshifting occurs due to the PK401 pseudoknot, translation will continue in the -1 frame to produce a CFP-YFP fusion protein with which FRET can be measured.
 This construct was designed to verify the applicability of frameshift inducing elements in the programming of protein expression ratios.