Difference between revisions of "Part:BBa K1159001"

Revision as of 01:38, 14 September 2013

NanoLuc Luciferase in RFC[25]

NanoLuc Luciferase is engineered ATP-independent luciferase from a deep-sea shrimp which luminescense 2 magnitues higher than these from Renilla reniformis or from Photinus pyralis (firefly). Also the molecular weight of NanoLuc luciferase is twice smaller compared to other luciferase (only 19 kDa).

Usage and Biology

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Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal XhoI site found at 10
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

Functional Parameters

Parameters for BioBrick BBa_K1159001 automatically created by the BioBrick-AutoAnnotator version 1.0

Nucleotide sequence in RFC 10: (underlined part encodes the protein)
ATGGCCGGC ... GCTACCGGTTAA
ORF from 1 to 525 (excluding stop-codon)

Amino acid sequence:
1 MAGVFTLEDFVGDWRQTAGYNLDQVLEQGGVSSLFQNLGVSVTPIQRIVLSGENGLKIDIHVIIPYEGLSGDQMGQIEKIFKVVYPVDDHHFKVILHYGT
101 LVIDGVTPNMIDYFGRPYEGIAVFDGKKITVTGTLWNGNKIIDERLINPDGSLLFRVTINGVTGWRLCERILATG

Amino acid composition:

A (Ala)	4 (2.3%)
R (Arg)	7 (4.0%)
N (Asn)	8 (4.6%)
D (Asp)	12 (6.9%)

C (Cys)	1 (0.6%)
Q (Gln)	7 (4.0%)
E (Glu)	8 (4.6%)
G (Gly)	22 (12.6%)

H (His)	4 (2.3%)
I (Ile)	18 (10.3%)
L (Leu)	16 (9.1%)
K (Lys)	7 (4.0%)

M (Met)	3 (1.7%)
F (Phe)	8 (4.6%)
P (Pro)	6 (3.4%)
S (Ser)	6 (3.4%)

T (Thr)	11 (6.3%)
W (Trp)	3 (1.7%)
Y (Tyr)	6 (3.4%)
V (Val)	18 (10.3%)

Amino acid counting:

	Total number:	175
	Positively charged (Arg+Lys):	14 (8.0%)
	Negatively charged (Asp+Glu):	20 (11.4%)

Biochemical parameters:

	Molecular mass [Da]:	19381.3
	Theoretical pI:	5.10
	Extinction coefficient at 280 nm [M^-1 cm^-1]:	25440 / 25503 (all Cys red/ox)

Organism specific parameters

Organism

E. coli

B. subtilis

S. cervisiae

A. thaliana

Mammals

Estimated half-life [h]

>10

Platzhalter

>20

Platzhalter

30

Codon usage (CAI)

good (0.66)

B. subtilis

good (0.62)

A. thaliana

excellent (0.82)

Organism specific parameters

Organism:	E. coli	B. subtilis	S. cervisiae	A. thaliana	Mammals
Estimated half-life [h]	>10	Platzhalter	>20	Platzhalter	30
Codon usage (CAI)	good (0.66)	B. subtilis	good (0.62)	A. thaliana	excellent (0.82)

Sequence features:

The BioBrick-AutoAnnotator was created by TU-Munich 2013 iGEM team. For more information please see the documentation.
If you have any questions, comments or suggestions, please email us at igem@wzw.tum.de.

Revision as of 15:13, 2 August 2013 (view source) Earthnut (Talk \| contribs) ← Older edit		Revision as of 01:38, 14 September 2013 (view source) Earthnut (Talk \| contribs) Newer edit →
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−	x	+	NanoLuc Luciferase is engineered ATP-independent luciferase from a deep-sea shrimp which luminescense 2 magnitues higher than these from ''Renilla reniformis'' or from ''Photinus pyralis'' (firefly). Also the molecular weight of NanoLuc luciferase is twice smaller compared to other luciferase (only 19 kDa).