Difference between revisions of "Part:BBa K1159003"

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<partinfo>BBa_K1159003 short</partinfo>
 
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{| class="wikitable" style="text-align: center; color: green;"
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|Orange
 +
|Apple
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|12,333.00
 +
|-
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|Bread
 +
|Pie
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|500.00
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|-
 +
|Butter
 +
|Ice cream
 +
|1.00
 +
|}
  
 
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Revision as of 16:03, 27 July 2013

Engineered Fluorescein-Binding Anticalin FluA (triple mutant variant) in RFC[25]

Orange Apple 12,333.00
Bread Pie 500.00
Butter Ice cream 1.00

Automatically determined parameters using the BioBrick-AutoAnnotator Version 1.0
Nucleotide sequence: (underlined part encodes the protein, italic parts were added)
 GTACACAATGCGTCGT ... TTCGAAAAATAA
Amino acid sequence:
  1 MRRSANYQPSIWDHDFLQSLNSNYTDEAYKRRAEELRGKVKIAIKDVIEPLDQLDLIDNLQRLGLAHRFETEIRNILNNIYNNNKDYNWRKENLYATSLE
101 FRLLRQHGYPVSQEVFNGFKDDQGGFICDDFKGILSLHEASYYSLEGESIMEEAWQFTSKHLKEVMISKNMEEDVFVAEQAKRALELPLHWKVPMLEARW
201 FIHIYERREDKNHLLLELAKMEFNTLQAIYQEELKEISGWWKDTGLGEKLSFARNRLVASFLWSMGIAFEPQFAYCRRVLTISIALITVIDDIYDVYGTL
301 DELEIFTDAVERWDINYALKHLPGYMKMCFLALYNFVNEFAYYVLKQQDFDLLLSIKNAWLGLIQAYLVEAKWYHSKYTPKLEEYLENGLVSITGPLIIT
401 ISYLSGTNPIIKKELEFLESNPDIVHWSSKIFRLQDDLGTSSDEIQRGDVPKSIQCYMHETGASEEVARQHIKDMMRQMWKKVNAYTADKDSPLTGTTTE
501 FLLNLVRMSHFMYLHGDGHGVQNQETIDVGFTLLFQPIPLEDKHMAFTASPGTKGTGAWSHPQFEK
BioBrick: BBa_K801060Used open reading frame from position 8 to 1705 (excluding stop-codon; if appropriate prefix/suffix were added).
Amino acid composition:
A (Ala)33 (5.8%)C (Cys)4 (0.7%)H (His)18 (3.2%)M (Met)16 (2.8%)T (Thr)26 (4.6%)
R (Arg)25 (4.4%)Q (Gln)24 (4.2%)I (Ile)39 (6.9%)F (Phe)28 (4.9%)W (Trp)14 (2.5%)
N (Asn)27 (4.8%)E (Glu)48 (8.5%)L (Leu)64 (11.3%)P (Pro)17 (3.0%)Y (Tyr)27 (4.8%)
D (Asp)34 (6.0%)G (Gly)29 (5.1%)K (Lys)36 (6.4%)S (Ser)33 (5.8%)V (Val)24 (4.2%)
Amino acid counting:Total number of amino acids (aa):566Number of positively charged aa (Arg + Lys):61Number of negatively charged aa (Asp + Glu):82
Biochemical parameters:Molecular weight [Da]:66105.33Theoretical pI:5.38Extinction coefficient at 280 nm [M-1 cm-1]:117480 (all Cys as cystine)
117230 (all Cys reduced)
Estimated half-life:Mammals:30 hYeast:>20 hE. coli:>10 h
Codon usage: (CAI)Mammals:0.68Yeast:0.69E. coli:0.71
RFC standard:This is a RFC 10 BioBrick, nothing was added
The BioBrick-AutoAnnotator was created by TU-Munich 2013 iGEM team. For information please read the description.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 248
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]