Difference between revisions of "Part:BBa J100114"

Latest revision as of 20:11, 17 July 2013

eCDM8 and GFP Riboswitch into pSB1A8

High promoter/RBS combination with eCDM8 (part J100101) plus promoter/riboswitch/GFP construct (J100079), ligated into pSB1A8 (J119043). This part was constructed using iPCR and PCR to amplify the desired sequences and add BsaI sites, and Golden Gate Assembly to ligate the sequences together. eCDM8 demethylates caffeine to produce theophylline, which in turn binds to the riboswitch, allowing the translation of GFP mRNA, producing green fluorescent protein as a reporter of theophylline production.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 237
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 196
Illegal AgeI site found at 857
Illegal AgeI site found at 3371
Illegal AgeI site found at 3455
1000
INCOMPATIBLE WITH RFC[1000]
Illegal SapI.rc site found at 3443

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 __NOTOC__
 <partinfo>BBa_J100114 short</partinfo>
-High promoter/RBS combination with eCDM8 (part J119303) plus promoter/riboswitch/GFP construct (J100079), ligated into pSB1A8 (J119043). This part was constructed using iPCR and PCR to amplify the desired sequences and add BsaI sites, and Golden Gate Assembly to ligate the sequences together. eCDM8 demethylates caffeine to produce theophylline, which in turn binds to the riboswitch, allowing the translation of GFP mRNA, producing green fluorescent protein as a reporter of theophylline production.
+High promoter/RBS combination with eCDM8 (part J100101) plus promoter/riboswitch/GFP construct (J100079), ligated into pSB1A8 (J119043). This part was constructed using iPCR and PCR to amplify the desired sequences and add BsaI sites, and Golden Gate Assembly to ligate the sequences together. eCDM8 demethylates caffeine to produce theophylline, which in turn binds to the riboswitch, allowing the translation of GFP mRNA, producing green fluorescent protein as a reporter of theophylline production.
 <!-- Add more about the biology of this part here