Difference between revisions of "Part:BBa K1051304"

 
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<partinfo>BBa_K1051304 short</partinfo>
 
<partinfo>BBa_K1051304 short</partinfo>
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<h3>Purpose</h3>
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It is a promoter which promotes transciption in M of the yeast cell cycle .If you want to express your proteins in M of the yeast cell cycle ,you can add the sequence which is actived by cln3  before your gene sequence ,then put them in a yeast plasmid .After transformation ,you can get the protein you want from the  metabolic product of the Bacterial you use for transformation .
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work to activates Cdc28p kinase to promote the G1 to S phase transition
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<br />
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<h3>Principle</h3>
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CLn3 is a promoter which promotes transciption in M of the yeast cell cycle .If you want to express your proteins in M of the yeast cell cycle ,you can add the sequence which is actived by cln3  before your gene sequence ,then put them in a yeast plasmid .After transformation ,you can get the protein you want from the  metabolic product of the Bacterial you use for transformation .it plays a role in regulating transcription of the other G1 cyclins, CLN1 and CLN2; regulated by phosphorylation and proteolysis; acetly-CoA induces CLN3 transcription in response to nutrient repletion to promote cell-cycle entry.
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 16:59, 27 September 2013

cln3 promoter (M phase to G1 phase)

Purpose

work to activates Cdc28p kinase to promote the G1 to S phase transition

Principle

CLn3 is a promoter which promotes transciption in M of the yeast cell cycle .If you want to express your proteins in M of the yeast cell cycle ,you can add the sequence which is actived by cln3 before your gene sequence ,then put them in a yeast plasmid .After transformation ,you can get the protein you want from the metabolic product of the Bacterial you use for transformation .it plays a role in regulating transcription of the other G1 cyclins, CLN1 and CLN2; regulated by phosphorylation and proteolysis; acetly-CoA induces CLN3 transcription in response to nutrient repletion to promote cell-cycle entry.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 661
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]