Difference between revisions of "Part:BBa K1065000:Design"

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<span style= "color:yellow" title="Prefix"><B>GAATTCGCGGCCGCTTCTAGA</B></span>TA<span style="color:purple; font-weight:bold" title="RBS">AGGAGG</span>AACTACTATG<span style="color:green" title="NgoMIV"><B>GCCGGC</B></span>ACC...<br/>
 
<span style= "color:yellow" title="Prefix"><B>GAATTCGCGGCCGCTTCTAGA</B></span>TA<span style="color:purple; font-weight:bold" title="RBS">AGGAGG</span>AACTACTATG<span style="color:green" title="NgoMIV"><B>GCCGGC</B></span>ACC...<br/>
 
<br/>
 
<br/>
−
TCG<span  style="color:red; font-weight:bold" title="AgeI">ACCGGT</span><b>TAA</b><span style="color:blue; font-weight:bold" title="suffix">TACTAGTAGCGGCCGCTGCAG</span>
+
TCG<span  style="color:red; font-weight:bold" title="AgeI">ACCGGT</span><b title="Stop">TAA</b><span style="color:blue; font-weight:bold" title="suffix">TACTAGTAGCGGCCGCTGCAG</span>
  
 
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Revision as of 11:54, 24 June 2013


2-oxoglutarate oxygenase/decarboxylase (EFE)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 319
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 17
    Illegal AgeI site found at 1070
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Ethylene-forming enzyme that catalyses the formation of ethylene from 2-oxoglutarate. The coding sequence was taken from Pseuhmonas syringa pv. phaseolicola PK2.


GAATTCGCGGCCGCTTCTAGATAAGGAGGAACTACTATGGCCGGCACC...

TCGACCGGTTAATACTAGTAGCGGCCGCTGCAG

Source

xxxx

References