Difference between revisions of "Help:Assembly Compatibility"
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| − | + | ===My part is not BioBrick compatible=== | |
| + | Before working with your part in the lab (characterizing/measureing), make sure it is BioBrick compatible. If not... | ||
| + | *'''[https://parts.igem.org/DNA_Synthesis Synthesize your part]''' to remove any illegal restriction sites, through synonymous substitutions (silent mutations). Synthesis is also useful, since you can synthesize your part with the BioBrick prefix and suffix, so it is ready for shipping to the Registry. [https://parts.igem.org/DNA_Synthesis See our synthesis offer with IDT] | ||
| + | *Use site-specific mutagenesis to remove any illegal restriction sites, through synonymous substitutions (silent mutations). | ||
Revision as of 16:25, 12 June 2014
My part is not BioBrick compatible
Before working with your part in the lab (characterizing/measureing), make sure it is BioBrick compatible. If not...
- Synthesize your part to remove any illegal restriction sites, through synonymous substitutions (silent mutations). Synthesis is also useful, since you can synthesize your part with the BioBrick prefix and suffix, so it is ready for shipping to the Registry. See our synthesis offer with IDT
- Use site-specific mutagenesis to remove any illegal restriction sites, through synonymous substitutions (silent mutations).