Difference between revisions of "Part:BBa K511811"
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1005123605 http://instructionsqsa.pp.ua/kml2.html http://manualiguj.pp.ua/tfhm1.html http://documents4xm7.pp.ua/ http://documentsjgq.pp.ua/bmy2.html http://manualip2cj.pp.ua/vnauw2.html http://manualiguj.pp.ua/evr2.html http://instructionsqsa.pp.ua/cfco1.html http://manualiguj.pp.ua/ http://documentsjgq.pp.ua/kuelta1.html http://documentsjgq.pp.ua/    http://manualip2cj.pp.ua/  http://documents140.pp.ua/  http://documents140.pp.ua/coyx1.html    http://documentspacs.pp.ua/                    http://documents140.pp.ua/rlivwk2.html
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__NOTOC__
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<partinfo>BBa_K511811 short</partinfo>
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This MammoBlock composite device produces the juxtacrine signaling Notch-Gal4-ESN receptor using the strong constitutive CMV promoter. This device is critical for cell patterning experiments employing the Notch-Delta system and is generally useful for the interrogation or engineering of multicellular phenomena that rely upon cell-cell contact.
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===Usage and Biology===
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<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K511811 SequenceAndFeatures</partinfo>
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===Functional Parameters===
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<partinfo>BBa_K511811 parameters</partinfo>
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Latest revision as of 16:18, 10 May 2013


Notch-Gal4-ESN Juxtacrine Receptor Generator (CMV-Notch-Gal4-ESN) MammoBlock Device

This MammoBlock composite device produces the juxtacrine signaling Notch-Gal4-ESN receptor using the strong constitutive CMV promoter. This device is critical for cell patterning experiments employing the Notch-Delta system and is generally useful for the interrogation or engineering of multicellular phenomena that rely upon cell-cell contact.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 6
    Illegal EcoRI site found at 601
    Illegal PstI site found at 881
    Illegal PstI site found at 1286
    Illegal PstI site found at 1616
    Illegal PstI site found at 1847
    Illegal PstI site found at 1944
    Illegal PstI site found at 3134
    Illegal PstI site found at 5012
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 6
    Illegal EcoRI site found at 601
    Illegal PstI site found at 881
    Illegal PstI site found at 1286
    Illegal PstI site found at 1616
    Illegal PstI site found at 1847
    Illegal PstI site found at 1944
    Illegal PstI site found at 3134
    Illegal PstI site found at 5012
    Illegal NotI site found at 5847
    Illegal NotI site found at 5855
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 6
    Illegal EcoRI site found at 601
    Illegal BamHI site found at 3556
    Illegal XhoI site found at 6138
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 6
    Illegal EcoRI site found at 601
    Illegal PstI site found at 881
    Illegal PstI site found at 1286
    Illegal PstI site found at 1616
    Illegal PstI site found at 1847
    Illegal PstI site found at 1944
    Illegal PstI site found at 3134
    Illegal PstI site found at 5012
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 6
    Illegal EcoRI site found at 601
    Illegal PstI site found at 881
    Illegal PstI site found at 1286
    Illegal PstI site found at 1616
    Illegal PstI site found at 1847
    Illegal PstI site found at 1944
    Illegal PstI site found at 3134
    Illegal PstI site found at 5012
    Illegal NgoMIV site found at 1147
    Illegal NgoMIV site found at 3259
    Illegal NgoMIV site found at 3315
    Illegal NgoMIV site found at 3358
    Illegal NgoMIV site found at 4143
    Illegal NgoMIV site found at 5160
    Illegal NgoMIV site found at 5337
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 6057
    Illegal BsaI.rc site found at 716
    Illegal BsaI.rc site found at 2424
    Illegal BsaI.rc site found at 3339
    Illegal BsaI.rc site found at 5796