Difference between revisions of "Part:BBa K511600"
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__NOTOC__
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<partinfo>BBa_K511600 short</partinfo>
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This part encodes a protein kmown to be instrumental in intercellular adhesion and tissue integrity. Like all cadherins, N-Cadherin mediates adhesion with five extracellular, calcium-binding repeats and an intercellular domain that makes contacts with the actin cytoskeleton of mammalian cells. This part additionally encodes an enhanced yellow fluorescent protein (EYFP) linked to the N-Cadherin by a self-cleaving viral 2A peptide, allowing for bicistronic expression of both a cell adhesion protein and an identifying marker with a single part.
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<!-- Add more about the biology of this part here
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===Usage and Biology===
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<!-- -->
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<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K511600 SequenceAndFeatures</partinfo>
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<!-- Uncomment this to enable Functional Parameter display
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===Functional Parameters===
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<partinfo>BBa_K511600 parameters</partinfo>
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<!-- -->

Latest revision as of 16:18, 10 May 2013


N-Cadherin-2A-EYFP Cell Adhesion MammoBlock

This part encodes a protein kmown to be instrumental in intercellular adhesion and tissue integrity. Like all cadherins, N-Cadherin mediates adhesion with five extracellular, calcium-binding repeats and an intercellular domain that makes contacts with the actin cytoskeleton of mammalian cells. This part additionally encodes an enhanced yellow fluorescent protein (EYFP) linked to the N-Cadherin by a self-cleaving viral 2A peptide, allowing for bicistronic expression of both a cell adhesion protein and an identifying marker with a single part.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 2723
    Illegal PstI site found at 287
    Illegal PstI site found at 1388
    Illegal PstI site found at 2999
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 2723
    Illegal PstI site found at 287
    Illegal PstI site found at 1388
    Illegal PstI site found at 2999
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 920
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 2723
    Illegal PstI site found at 287
    Illegal PstI site found at 1388
    Illegal PstI site found at 2999
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 2723
    Illegal PstI site found at 287
    Illegal PstI site found at 1388
    Illegal PstI site found at 2999
    Illegal NgoMIV site found at 1239
    Illegal NgoMIV site found at 2765
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1274
    Illegal BsaI.rc site found at 1831