Difference between revisions of "Test2"

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|Photinus/Firefly luciferase
 
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|D-luciferin + ATP
 
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|Renilla luciferase
 
|Renilla luciferase
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|coelenterazine + O2
 
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|Gaussia luciferase
 
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|coelenterazine + O2
 
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|Nano luciferase
 
|Nano luciferase
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|furimazine
 
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Revision as of 22:28, 6 March 2013

Icon Sensors.png


Biosafety: Parts improving biological safety, containment.
Biosynthesis: Parts involved in the production of chemicals and metabolites are listed here.
Biodegradation: Parts involved in the degradation of chemicals are listed here.
Cell-cell signaling and quorum sensing: Parts involved in intercellular signaling and quorum sensing between bacteria.
Cell death: Parts involved in killing cells.
Coliroid: Parts involved in taking a bacterial photograph.
Conjugation: Parts involved in DNA conjugation between bacteria.
DNA binding: Parts involved in xxx.
DNA recombination: Parts involved in DNA recombination are listet here.
Ions: Parts involved in sensing, transport and binding of different ions are listet in here.
Ligand Binding: Parts involved in binding chemical structures are listet here.
Light production and sensing: Parts involved in generation and sensing of light are listet here.
Motility and chemotaxis: Parts involved in motility or chemotaxis of cells are listet here.
Odor production and sensing: Parts that produce or sense odorants aer listet here.
Sensors: Parts used as biosensors are listed here.
Viral vectors: Parts involved in the production and modification of Viral vectors are listet here..












Fluorescent Proteins

Featured Parts, [http://www.microscopyu.com/articles/livecellimaging/fpintro.html Nikkon], [http://2012.igem.org/Team:Uppsala_University/Chromoproteins Chromoproteins Uppsala] https://parts.igem.org/Part:BBa_K180009 mBanana

Name Exitation [nm] Emmission [nm] Quant. yield Molar extinct. BioBrick RFC10 BioBrick RFC25 Reference
Cerulean 433 475 0.62 43000 BBa_E2020
GFPmut3b 501 511 BBa_E0040
amilGFP 503 512 0.67 75200 BBa_K592010 [http://www.ncbi.nlm.nih.gov/nuccore/ay646067 GeneBank]
mVenus 515 528 0.57 92200 BBa_I757008
mOrange 548 562 0.69 71000 BBa_E2050
mStrawbery 574 596 0.29 90000 BBa_K399001
mRFP1 584 607 0.25 50000 BBa_E1010
mCherry 587 610 0.22 72000 BBa_E2060
eforRed 589 609 0.16 111300 BBa_K592012 [http://www.ncbi.nlm.nih.gov/nuccore/EU498726 GeneBank]
cjBlue 610 BBa_K592011 [http://www.ncbi.nlm.nih.gov/pubmed/17028187 PubMed]
aeBlue BBa_K864401 [http://www.ncbi.nlm.nih.gov/pubmed/16164420 PubMed]
amilCP 588 NA NA 8760 BBa_K592009 [http://www.ncbi.nlm.nih.gov/nuccore/AY646075 GeneBank]


Luciferases

Name Organism Substrate pH optimum Amino acids Mol. mass [kDa] Emmission [nm] BioBrick RFC10 BioBrick RFC25 Reference
Photinus/Firefly luciferase D-luciferin + ATP 550 60.6 578 BBa_I712019
Renilla luciferase coelenterazine + O2 311 36.0 480 BBa_J52008
Gaussia luciferase coelenterazine + O2 189 20.3 480 BBa_K509005
Nano luciferase furimazine 171 19.1 460 [http://www.reportergene.com/2013/01/the-nanoluc-luciferase-reporter-dossier.html 1]

Photoproteins

(Aequorin, Obelin