Difference between revisions of "Add a Part to the Registry"

Revision as of 15:45, 27 July 2006

Just starting? Need help? Check out our documentation on How to make a Biobrick!
Or maybe you're looking for how to standardize a non-Biobrick sequence before you add it?

Members of Registry groups may add three kinds of parts to the registry: Basic Parts, Composite Parts, and Construction Intermediates.

Basic Parts Add a Basic Part Now...

Basic Parts are atomic units of DNA. They cannot be subdivided into smaller component parts. DNA for a basic part may be obtained by de-novo synthesis, genbank, primer extension and PCR, or other techniques. Like all parts, a Basic Part is stored in a plasmid, surrounded by a restriction-enzyme cloning region ("BioBrick ends"). This cloning region is not included in the sequence of the part. It can be provided by the Registry software. Here is an example of a Basic Part.

Composite Parts Add a Composite Part Now...

Composite Parts are functional units made from an ordered series of basic parts or other composite parts. Explicit base pairs of DNA cannot be entered in as sequence for these parts (parts which do require you to manually enter sequence are Basic Parts). The Registry's software provides information and sequence for all the basic parts that you list as components of your composite part. While the Registry provides the sequence from the component parts specified, the function and design issues of the composite part should be documented in detail. Here is an example of a Composite Part.

Construction Intermediates Add a Construction Intermediate Now...

Construction Intermediates have no specific function and are just the result of assembling two parts together. They require no further documentation. Often they are unwanted byproducts of construction They all have the type 'Intermediate' and part names of the form 'BBa_Snnnnn'. These part names are automatically assigned by the Registry software. Once you enter your intermediate part in the Registry, you will be able to use BioBrick Blast to check your assembly's sequence and your part will show up in the subpart and superpart search functions. If you send us the DNA, we will be able to share your work with others and include it in assemblies done by the Registry. There are no examples of these parts available yet.

The most important feature of a standard biological part should be that a user of the part does not have to talk to you, the designer of the part. This is achieved by completely documenting the part.