Difference between revisions of "Part:BBa M36071"

Revision as of 11:07, 7 December 2012

K12 e.coli native Manganese Superoxide Dismutase

Coding sequence for the enzyme Mn-SOD or SoDA. This protein catalyzes the dismutation of superoxide, a free radical. Sequence found by converting the amino acid sequence of Mn-SOD protein native to Escherichia coli, the organism for which it is also optimized for.

Usage and Biology

Superoxide dismutase cataylyzes the dismutation of superoxide. Superoxide is produced naturally in the respiration process. It causes damage to the cell by altering lipid structure and breaking down proteins among other negative effects.

Manganese Superoxide Dismutase is one of two superoxide dismutase enzymes that e. coli naturally produces

Design Notes

This DNA sequence is converted from the amino acid sequence of Mn-SOD protein using the online converter from In-Silico. We optimized our sequence in DNA 2.0's Gene Designer by increasing GC content and minimizing repeats. We accomplished this by altering basepairs without altering amino acids.

Source

This sequence is derived from the amino acid sequence of Mn-SOD protein found from Durfee et. al at the University of Wisconsin which also provided the entire genomic sequence of the E. coli (strain: k-12, substrain: DH10B) genome.

Keele Jr, Bernard B., J. M. McCord, and I. Fridovich. "Superoxide dismutase from Escherichia coli BA new manganese-containing enzyme." Journal of Biological Chemistry 245.22 (1970): 6176-6181.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal EcoRI site found at 346
Illegal PstI site found at 283
Illegal PstI site found at 593
12
INCOMPATIBLE WITH RFC[12]
Illegal EcoRI site found at 346
Illegal PstI site found at 283
Illegal PstI site found at 593
21
INCOMPATIBLE WITH RFC[21]
Illegal EcoRI site found at 346
Illegal XhoI site found at 43
Illegal XhoI site found at 127
23
INCOMPATIBLE WITH RFC[23]
Illegal EcoRI site found at 346
Illegal PstI site found at 283
Illegal PstI site found at 593
25
INCOMPATIBLE WITH RFC[25]
Illegal EcoRI site found at 346
Illegal PstI site found at 283
Illegal PstI site found at 593
Illegal NgoMIV site found at 542
1000
COMPATIBLE WITH RFC[1000]

@@ Line 2: / Line 2: @@
 <partinfo>BBa_M36071 short</partinfo>
-Coding sequence for the enzyme Mn-SOD. This protein catalyzes the dismutation of superoxide, a free radical. Sequence found by converting the amino acid sequence of Mn-SOD protein native to Escherichia coli, the organism for which it is also optimized for.
+Coding sequence for the enzyme Mn-SOD or SoDA. This protein catalyzes the dismutation of superoxide, a free radical. Sequence found by converting the amino acid sequence of Mn-SOD protein native to Escherichia coli, the organism for which it is also optimized for.
 ===Usage and Biology===
 Superoxide dismutase cataylyzes the dismutation of superoxide. Superoxide is produced naturally in the respiration process. It causes damage to the cell by altering lipid structure and breaking down proteins among other negative effects.
+Manganese Superoxide Dismutase is one of two superoxide dismutase enzymes that e. coli naturally produces
 ===Design Notes===
@@ Line 15: / Line 17: @@
 This sequence is derived from the amino acid sequence of Mn-SOD protein found from Durfee et. al at the University of Wisconsin which also provided the entire genomic sequence of the E. coli (strain: k-12, substrain: DH10B) genome.
+Keele Jr, Bernard B., J. M. McCord, and I. Fridovich. "Superoxide dismutase from Escherichia coli BA new
+manganese-containing enzyme." Journal of Biological Chemistry 245.22 (1970): 6176-6181.
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