Difference between revisions of "Part:BBa K902084"

Revision as of 06:42, 28 October 2012

Prha-B0034-S7(active)

This part the Prha promoter, which is repressed by glucose and induced by rhamnose upstream of S7 micrococcal nuclease. This is an active killswitch where upon induction with rhamnose, the nuclease is produced, resulting in cell death. Conversely, when glucose is added, the promoter is repressed, causing no expression of the nuclease gene, resulting in the cells being able to grow. We characterized this circuit using differing concentration of glucose and rhamnose. The results show that with increasing concentrations of glucose, this killswitch is deactivated. The Prha promoter was initially characterized via fluorescense using GFP. This data can be found here. Please see our Wiki for more information!

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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 <partinfo>BBa_K902084 short</partinfo>
-<html>This part has the Prha promoter with S7 micrococcal nuclease. This is an active killswitch. With increasing concentrations of glucose this killswitch is deactivated.
+<html>This part the Prha promoter, which is repressed by glucose and induced by rhamnose upstream of <i>S7 micrococcal nuclease</i>. This is an active killswitch where upon induction with rhamnose, the nuclease is produced, resulting in cell death.  Conversely, when glucose is added, the promoter is repressed, causing no expression of the nuclease gene, resulting in the cells being able to grow.  We characterized this circuit using differing concentration of glucose and rhamnose.  The results show that with increasing concentrations of glucose, this killswitch is deactivated.
-Please see <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K902066">for GFP data</a>. Please see our <a href="http://2012.igem.org/Team:Calgary/Project/Synergy">Wiki</a> for data.</html>
+The Prha promoter was initially characterized via fluorescense using GFP.  This data can be found  <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K902066">here</a>. Please see our <a href="http://2012.igem.org/Team:Calgary/Project/Synergy">Wiki</a> for more information!</html>
 [[Image:Calgary Rha S7 Data.png|thumb|500px|center]]