Difference between revisions of "Part:BBa K812012"
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This part can be micro-injected using pSC2+ ( <html><a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K812000">BBa_K812000</a></html>) or related plasmid as a backbone. | This part can be micro-injected using pSC2+ ( <html><a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K812000">BBa_K812000</a></html>) or related plasmid as a backbone. | ||
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| + | <img src="https://static.igem.org/mediawiki/parts/5/5a/Degron1.jpg" alt="perdu" width="1000px" /> <br/><br/> | ||
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Latest revision as of 00:10, 27 October 2012
OsTirI Ubiquitinase E3 for AID tagged protein degradation in the presence of auxin
OsTiRI is an ubuquitinase E3 that recognizes AID-tagged proteins and trigger their ubiquitination for further degradation. This part works with K812011 which is a AID tagged GFP. OsTirI is a protein comming from rice that have been identified as working in mammalian cells and yeast.
This part takes place in a device pattented by Kanemaki Masato, Kakimoto Tatsuo, Nishimura Kohei, Takisawa Haruhiko and Fukagawa Tatsuo for yeast and mammalian cells use (http://www.freepatentsonline.com/y2012/0115232.html). However the patent does not cover the use for oviparian such as frogs and chicken.
This part contains 3 myc domains in C-terminus for protein staining using antiMyc antibodies.
This part can be micro-injected using pSC2+ ( BBa_K812000) or related plasmid as a backbone.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 433
Illegal NotI site found at 287 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1648
Illegal XhoI site found at 356 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 55
Illegal NgoMIV site found at 164 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 79