Difference between revisions of "Part:BBa K902041:Experience"
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===Applications of BBa_K902041=== | ===Applications of BBa_K902041=== | ||
− | <html><i>amdA</i> is an Amidase gene responsible for the removal of primary | + | <html><i>amdA</i> is an Amidase gene responsible for the removal of primary amides from a variety of compounds. We validated the ability of this gene to remove the primary amide group from benzamide. This was evaluated through GC/MS analysis and detailed on our <a href="http://2012.igem.org/Team:Calgary/Notebook/Protocols/AmidaseAssay">protocol page</a>. This is detailed in the figures below: |
</html>[[File:Calgary AmdA Chromatograph.png|thumb|320px|left|Figure 1: GC chromatograph demonstrating the degradation of benzamide by <i>AmdA</i> compared to an <i>E. coli</i> control. Additionally, a smaller peak with an earlier retention time is produced solely in the sample run.]]<html> | </html>[[File:Calgary AmdA Chromatograph.png|thumb|320px|left|Figure 1: GC chromatograph demonstrating the degradation of benzamide by <i>AmdA</i> compared to an <i>E. coli</i> control. Additionally, a smaller peak with an earlier retention time is produced solely in the sample run.]]<html> |
Revision as of 19:53, 26 October 2012
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Applications of BBa_K902041
amdA is an Amidase gene responsible for the removal of primary amides from a variety of compounds. We validated the ability of this gene to remove the primary amide group from benzamide. This was evaluated through GC/MS analysis and detailed on our protocol page. This is detailed in the figures below:
===User Reviews===