Difference between revisions of "Part:BBa K902041:Experience"

Revision as of 09:59, 26 October 2012

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Applications of BBa_K902041

amdA is an Amidase gene responsible for the removal of primary amine's from a variety of compounds. We validated the ability of this gene to remove the primary amine group from benzamine. This was evaluated through GC/MS analysis and detailed on our <a href="http://2012.igem.org/Team:Calgary/Notebook/Protocols/AmidaseAssay">protocol page</a>. This is detailed in the figures below:

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Figure 1: GC chromatograph demonstrating the degradation of benzamide by AmdA compared to an E. coli control. Additionally, a smaller peak with an earlier retention time is produced solely in the sample run.

Figure 2: Mass Spectra for the peak at a retention time of 10.5 minutes from Figure 1 representing the substrate benzamine. Additionally, the peak at retention time of 9 minutes represents benzoic acid based on its mass suggesting that their is a small accumulation of product.

===User Reviews=== BBa_K902041 StartReviews BBa_K902041 EndReviews

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 ===Applications of BBa_K902041===
+<i>amdA</i> is an Amidase gene responsible for the removal of primary amine's from a variety of compounds.  We validated the ability of this gene to remove the primary amine group from benzamine.  This was evaluated through GC/MS analysis and detailed on our <a href="http://2012.igem.org/Team:Calgary/Notebook/Protocols/AmidaseAssay">protocol page</a>.  This is detailed in the figures below:
+</html>[[File:Calgary AmdA Chromatograph.png|thumb|320px|left|Figure 1: GC chromatograph demonstrating the degradation of benzamide by <i>AmdA</i> compared to an <i>E. coli</i> control.  Additionally, a smaller peak with an earlier retention time is produced solely in the sample run.]]<html>
+</html>[[File:Calgary AmdA MSData.png|thumb|320px|right|Figure 2: Mass Spectra for the peak at a retention time of 10.5 minutes from Figure 1 representing the substrate benzamine.  Additionally, the peak at retention time of 9 minutes represents benzoic acid based on its mass suggesting that their is a small accumulation of product.]]<html>
 ===User Reviews===
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 <I>Calgary</I>
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-<i>amdA</i> is an Amidase gene responsible for the removal of primary amine's from a variety of compounds.  We validated the ability of this gene to remove the primary amine group from benzamine.  This was evaluated through GC/MS analysis and detailed on our <a href="http://2012.igem.org/Team:Calgary/Notebook/Protocols/AmidaseAssay">protocol page</a>.  This is detailed in the figures below:
+|};
-</html>[[File:Calgary AmdA Chromatograph.png|thumb|320px|left|Figure 1: GC chromatograph demonstrating the degradation of benzamide by <i>AmdA</i> compared to an <i>E. coli</i> control.  Additionally, a smaller peak with an earlier retention time is produced solely in the sample run.]]<html>
-</html>[[File:Calgary AmdA MSData.png|thumb|320px|right|Figure 2: Mass Spectra for the peak at a retention time of 10.5 minutes from Figure 1 representing the substrate benzamine.  Additionally, the peak at retention time of 9 minutes represents benzoic acid based on its mass suggesting that their is a small accumulation of product.]]<html>|};
 <!-- End of the user review template -->
 <!-- DON'T DELETE --><partinfo>BBa_K902041 EndReviews</partinfo>