Difference between revisions of "Part:BBa K902041:Experience"
Rpgguardian (Talk | contribs) |
Rpgguardian (Talk | contribs) |
||
| Line 12: | Line 12: | ||
|width='10%'| | |width='10%'| | ||
<partinfo>BBa_K902041 AddReview 1</partinfo> | <partinfo>BBa_K902041 AddReview 1</partinfo> | ||
| − | <I> | + | <I>Calgary</I> |
|width='60%' valign='top'| | |width='60%' valign='top'| | ||
<i>amdA</i> is an Amidase gene responsible for the removal of primary amine's from a variety of compounds. We validated the ability of this gene to remove the primary amine group from benzamine. This was evaluated through GC/MS analysis and detailed on our <a href="http://2012.igem.org/Team:Calgary/Notebook/Protocols/AmidaseAssay">protocol page</a>. This is detailed in the figures below: | <i>amdA</i> is an Amidase gene responsible for the removal of primary amine's from a variety of compounds. We validated the ability of this gene to remove the primary amine group from benzamine. This was evaluated through GC/MS analysis and detailed on our <a href="http://2012.igem.org/Team:Calgary/Notebook/Protocols/AmidaseAssay">protocol page</a>. This is detailed in the figures below: | ||
| Line 18: | Line 18: | ||
</html>[[File:Calgary AmdA Chromatograph.png|thumb|320px|left|Figure 1: GC chromatograph demonstrating the degradation of benzamide by <i>AmdA</i> compared to an <i>E. coli</i> control. Additionally, a smaller peak with an earlier retention time is produced solely in the sample run.]]<html> | </html>[[File:Calgary AmdA Chromatograph.png|thumb|320px|left|Figure 1: GC chromatograph demonstrating the degradation of benzamide by <i>AmdA</i> compared to an <i>E. coli</i> control. Additionally, a smaller peak with an earlier retention time is produced solely in the sample run.]]<html> | ||
| − | </html>[[File:Calgary AmdA MSData.png|thumb|320px|right|Figure 2: Mass Spectra for the peak at a retention time of 10.5 minutes from Figure | + | </html>[[File:Calgary AmdA MSData.png|thumb|320px|right|Figure 2: Mass Spectra for the peak at a retention time of 10.5 minutes from Figure 1 representing the substrate benzamine. Additionally, the peak at retention time of 9 minutes represents benzoic acid based on its mass suggesting that their is a small accumulation of product.]]<html>|}; |
<!-- End of the user review template --> | <!-- End of the user review template --> | ||
<!-- DON'T DELETE --><partinfo>BBa_K902041 EndReviews</partinfo> | <!-- DON'T DELETE --><partinfo>BBa_K902041 EndReviews</partinfo> | ||
Revision as of 09:58, 26 October 2012
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_K902041
User Reviews
UNIQd9b91aebdbe57047-partinfo-00000000-QINU UNIQd9b91aebdbe57047-partinfo-00000001-QINU