Difference between revisions of "Part:BBa K883705"

 
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[[Image:GNC4_homodimer.jpg|800px|thumb|<p align="justify">''Figure 1: cross-section view of a E-/ K-coil heterodimer'</p>]]
  
 
__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K883705 short</partinfo>
 
<partinfo>BBa_K883705 short</partinfo>
  
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GFP with His tag at the C-terminus and the E-coil at the N-terminus behind an IPTG induced promoter to use it as reporter for the E- and K-coil Plug and Apply system
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GFP with His tag at the C-terminus and the E-coil at the N-terminus behind an IPTG induced promoter to use it as reporter for the E- and K-coil [http://2012.igem.org/Team:Wageningen_UR/Coil_system Plug and Apply system]
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===Usage and Biology===
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<p align="justify">
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The E-coil present in this reporter protein is one part of the Plug and Apply (PnA) system consisting of a combination of E- and K-coil. These coils consist of α-helical repeats and have a strong affinity to each other due to electrostatic interactions (with K-coil positively charged and E-coil negatively charged).
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Using this system one can bind two proteins noncovalently but specifically to each other.
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This brick can be used for confirmation of the Plug and Apply system using E- and K-coils as well as for detection of proteins that can be fused to the K-coil.
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</p>
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[[Image:Gfpcoilhistag.PNG|300px|center|thumb|<p align="justify">''Figure 4: protein folding prediction of GFP (green) with a His-tag (blue) fused to a coiled coil (purple)]]
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K883705 SequenceAndFeatures</partinfo>
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<partinfo>BBa_K883703 SequenceAndFeatures</partinfo>
  
  
 
<!-- Uncomment this to enable Functional Parameter display  
 
<!-- Uncomment this to enable Functional Parameter display  
 
===Functional Parameters===
 
===Functional Parameters===
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<partinfo>BBa_K883705 parameters</partinfo>
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<partinfo>BBa_K883703 parameters</partinfo>
 
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Revision as of 14:23, 26 October 2012

Figure 1: cross-section view of a E-/ K-coil heterodimer'


IPTG induced promoter + GFP with a His-tag fused to a coiled coil

GFP with His tag at the C-terminus and the E-coil at the N-terminus behind an IPTG induced promoter to use it as reporter for the E- and K-coil [http://2012.igem.org/Team:Wageningen_UR/Coil_system Plug and Apply system]

Usage and Biology

The E-coil present in this reporter protein is one part of the Plug and Apply (PnA) system consisting of a combination of E- and K-coil. These coils consist of α-helical repeats and have a strong affinity to each other due to electrostatic interactions (with K-coil positively charged and E-coil negatively charged). Using this system one can bind two proteins noncovalently but specifically to each other. This brick can be used for confirmation of the Plug and Apply system using E- and K-coils as well as for detection of proteins that can be fused to the K-coil.

Figure 4: protein folding prediction of GFP (green) with a His-tag (blue) fused to a coiled coil (purple)


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 930