Difference between revisions of "Part:BBa K801020:Design"
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* Cloned from genomic DNA of ''Kluyveromyces lactis'' strain WSYC115 | * Cloned from genomic DNA of ''Kluyveromyces lactis'' strain WSYC115 | ||
* '''Forward Primer:'''<br><code>5'- AGT CAG TCT AGA ACG ACG GTA CAT AAG AAT GAG G - 3'</code><br> | * '''Forward Primer:'''<br><code>5'- AGT CAG TCT AGA ACG ACG GTA CAT AAG AAT GAG G - 3'</code><br> | ||
| − | + | * '''Reverse Primer:'''<br><code>5'- GGC TAA GGT ACC CAT ATG GTG TGT TGT ATG TTG | |
| − | TGT TGT TGG - 3'</code><br | + | TGT TGT TGG - 3'</code><br> |
'''Organism:'''<br> | '''Organism:'''<br> | ||
| − | * | + | * Sequence derived from ''Kluyveromyces lactis'' |
<!--*Codonoptimized for ''?organism_name?''--> | <!--*Codonoptimized for ''?organism_name?''--> | ||
<!--*Designed for the following Chassis: ''?organism-name?''--> | <!--*Designed for the following Chassis: ''?organism-name?''--> | ||
<!--*Statement about functionality in other chassis.--> | <!--*Statement about functionality in other chassis.--> | ||
| − | |||
===References=== | ===References=== | ||
Revision as of 15:48, 22 October 2012
KlADH4 yeast promoter, ethanol inducible
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 753
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 590
To remove a naturally occuring SpeI-restriction site, the naturally occuring "A" at position 757 was changed to "T" using quickchange mutagenesis.
The feature annotation in the UASe region (Upstream activating sequence "ethanol") is taken from:
- Mazzoni, C., Santori, F., Saliola, M. & Falcone, C. (2000) ‚Molecular analysis of UASE, a cis element containing stress response elements responsible for ethanol induction of the KlADH4 gene of Kluyveromyces lactis’, Res. Microbiol. 151, 19-28. PMID: 10724480
Extracted from genomic DNA of K. lactis using PCR.
Keywords:
ethanol inducible, KlADH4, yeast, promoter, inducible, K. lactis, S. cerevisiae
Abbreviations:
Design Notes
Related BioBrick:
- Other versions:BBa_K678001: PalcA, inducible Aspergillus nidulans promoter
- Part was totally sequenced
Backbone:
- Backbone name: pSB1C3'
- Resistance: Cam
- Copynumber: high
Protein coding:
-
Enzymatic activity: -
Cytotoxicity:
- none
Safety notes:
- Known and anticipated sefety issues: none
- Known and anticipated security issues: none
Intellectual property:
Corresponding part author/authors:
Source
Source:
- Cloned from genomic DNA of Kluyveromyces lactis strain WSYC115
- Forward Primer:
5'- AGT CAG TCT AGA ACG ACG GTA CAT AAG AAT GAG G - 3' - Reverse Primer:
5'- GGC TAA GGT ACC CAT ATG GTG TGT TGT ATG TTG
TGT TGT TGG - 3'
Organism:
- Sequence derived from Kluyveromyces lactis
References
Literature references:
- [1] Saliola, M., Mazzoni, C., Solimando, N., Crisà, A., Falcone, C. & Jung, G. (1999) ‚Use of the KlADH4 promoter for ethanol-dependent production of recombinant human serum albumin in Kluyveromyces lactis’, Appl Environ Microbiol. 65 (1), 53-60. [http://www.ncbi.nlm.nih.gov/pubmed?term=10724480 PMID: 9872759]
- [2] Mazzoni, C., Santori, F., Saliola, M. & Falcone, C. (2000) ‚Molecular analysis of UASE, a cis element containing stress response elements responsible for ethanol induction of the KlADH4 gene of Kluyveromyces lactis’, Res. Microbiol. 151, 19-28. [http://www.ncbi.nlm.nih.gov/pubmed?term=10724480 PMID: 10724480]