Difference between revisions of "Part:BBa K814000"
Line 10: Line 10:
  
 
Figure 1.  Colony PCR screen confirming cloning of DHQS into E. coli pUCBB plasmid.
 
Figure 1.  Colony PCR screen confirming cloning of DHQS into E. coli pUCBB plasmid.
 +
 +
Balskus and Walsh, 2010. [http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3116657/]
  
  

Revision as of 22:53, 13 October 2012

dehydroquinate synthase (DHQS) generator

Our research has focused on two novel biosynthetic pathways found in two distinct algal species. A pathway ending in the production of two UV-protective compounds, shinorine and mycosporine-glycine, was cloned from Anabaena varibalis. DHQS catalyzes the first step in the pathway, converting sedoheptulose-7-phosphate into dehydroquinate.

This part includes a modified constitutive lac promoter (lacP'), and RBS and the open reading frame of DHQS. This part can be used to express DHQS in E. coli.


Dhqs.png

Figure 1. Colony PCR screen confirming cloning of DHQS into E. coli pUCBB plasmid.

Balskus and Walsh, 2010. [http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3116657/]


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 250
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 250
    Illegal NotI site found at 1373
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 250
    Illegal BglII site found at 134
    Illegal XhoI site found at 1381
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 250
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 250
  • 1000
    COMPATIBLE WITH RFC[1000]