Difference between revisions of "Part:BBa K779307"
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| − | <i> We transfected 250 ng of Hef1A:TagBFP into 100,000 HEK293 cells using Lipofectamine 2000 reagent. Images were taken on a Leica Confocal microscope at 10X. Left shows a brightfield image. Right shows the blue channel </i> | + | <i> We transfected 250 ng of Hef1A:TagBFP into 100,000 HEK293 cells using Lipofectamine 2000 reagent. Images were taken on a Leica Confocal microscope at 10X. Left shows a brightfield image. Right shows the blue channel. We can observe high transfection efficiency and expression of TagBFP. </i> |
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Latest revision as of 03:29, 11 October 2012
TagBFP-FF5 MammoBlock
We appended microRNA binding sites to TagBFP, an optimized Blue Fluorescent Protein.
We transfected 250 ng of Hef1A:TagBFP into 100,000 HEK293 cells using Lipofectamine 2000 reagent. Images were taken on a Leica Confocal microscope at 10X. Left shows a brightfield image. Right shows the blue channel. We can observe high transfection efficiency and expression of TagBFP.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NotI site found at 89
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 722