Difference between revisions of "Part:BBa K932000"
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| − | Controlled by the tet repressible promoter, the EnvZ protein expresses constitutively in the absence of the TetR protein. EnvZ is a phosphorylase kinase involved in activation of OmpR, a transcription factor. OmpR activates the expression of the csgD operon, which codes for the curli proteins, fibrous filaments involved in cell-surface and cell-cell adhesion. We expect expression of this part to enhance transcriptional activation of the curli proteins. Cells containing this construct should aggregate more rapidly, thus becoming more amenable to the formation of biofilms. | + | Controlled by the tet repressible promoter, the EnvZ protein expresses constitutively in the absence of the TetR protein. EnvZ is a phosphorylase kinase involved in activation of OmpR, a transcription factor. OmpR activates the expression of the csgD operon, which codes for the curli proteins. These enzymes, once expressed on the outer membrane, assemble fibrous filaments involved in cell-surface and cell-cell adhesion. We expect expression of this part to enhance transcriptional activation of the curli proteins and consequent filament assembly. Cells containing this construct should aggregate more rapidly, thus becoming more amenable to the formation of biofilms. |
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Revision as of 14:04, 6 October 2012
OmpR234 under Tet Repressible Promoter
This part promotes the constitutive expression of the curli cluster (csgD), proteins involved in cell-cell adhesion.
Usage and Biology
Controlled by the tet repressible promoter, the EnvZ protein expresses constitutively in the absence of the TetR protein. EnvZ is a phosphorylase kinase involved in activation of OmpR, a transcription factor. OmpR activates the expression of the csgD operon, which codes for the curli proteins. These enzymes, once expressed on the outer membrane, assemble fibrous filaments involved in cell-surface and cell-cell adhesion. We expect expression of this part to enhance transcriptional activation of the curli proteins and consequent filament assembly. Cells containing this construct should aggregate more rapidly, thus becoming more amenable to the formation of biofilms.
SEM images of E. coli BL-21 DE3 cells not expressing (left) and expressing (right) BBa_K932000. Expression qualitatively enhances cellular aggregation.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]