Difference between revisions of "Part:BBa K817050"
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Before start, we incubate the E. coli with promoter P<sub>hs</sub> and mRFP as a reporter at room temperature 25 Celsius degree. We transient shift the E. coli to a higher temperature ambient (37 Celsius degree), and closely monitor the expression of the reporter. As we can see in the figure, the promoter responds to the temperature changes pretty quick and after 30 minutes, it show 6 fold augmentation in mRFP emission (wavelength 610 nm). We continue the test and tendency of increment shows no sign of subsidence. | Before start, we incubate the E. coli with promoter P<sub>hs</sub> and mRFP as a reporter at room temperature 25 Celsius degree. We transient shift the E. coli to a higher temperature ambient (37 Celsius degree), and closely monitor the expression of the reporter. As we can see in the figure, the promoter responds to the temperature changes pretty quick and after 30 minutes, it show 6 fold augmentation in mRFP emission (wavelength 610 nm). We continue the test and tendency of increment shows no sign of subsidence. | ||
| − | <center><html><img src='https://static.igem.org/mediawiki/2012/9/91/NTU-Taida-Result-Thermal-phs.png' width=' | + | <center><html><img src='https://static.igem.org/mediawiki/2012/9/91/NTU-Taida-Result-Thermal-phs.png' width='600px'></html></center> |
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Latest revision as of 11:58, 5 October 2012
Novel thermal promoter, Phs
Phs, a thermal promoter, described by Wayne E. Taylor in Cell Press, 1984, can provide quick response to temperature upshift.
Description
Promoter Phs is a novel thermal response designed by Wayne E Taylor et. al; it is super sensitive to temperature increase. We would expect to see the sudden increase in expression of the reporter gene, in this case, mRFP.
Before start, we incubate the E. coli with promoter Phs and mRFP as a reporter at room temperature 25 Celsius degree. We transient shift the E. coli to a higher temperature ambient (37 Celsius degree), and closely monitor the expression of the reporter. As we can see in the figure, the promoter responds to the temperature changes pretty quick and after 30 minutes, it show 6 fold augmentation in mRFP emission (wavelength 610 nm). We continue the test and tendency of increment shows no sign of subsidence.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]