Difference between revisions of "Part:BBa K206009:Experience"
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As shown above, the digestion by P linearizes the plasmid as expected but X and S have no effect compared to the non digested plasmid. Moreover, double digestion by X+P only linearizes the plasmid. | As shown above, the digestion by P linearizes the plasmid as expected but X and S have no effect compared to the non digested plasmid. Moreover, double digestion by X+P only linearizes the plasmid. | ||
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<html><p> The kit plate DNA of this part from Spring 2012 distribution did not work in the hands of the 2012 iGEM Calgary part. University of Calgary 2012 iGEM team built BBa_B0034 and BBa_K082003 and resubmitted this part as <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K902016">BBa_K902016</a>.</html> | <html><p> The kit plate DNA of this part from Spring 2012 distribution did not work in the hands of the 2012 iGEM Calgary part. University of Calgary 2012 iGEM team built BBa_B0034 and BBa_K082003 and resubmitted this part as <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K902016">BBa_K902016</a>.</html> | ||
Revision as of 02:48, 4 October 2012
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_K206009
User Reviews
UNIQ2327e38110a5c245-partinfo-00000000-QINU UNIQ2327e38110a5c245-partinfo-00000001-QINU
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Alex273 |
The plasmid seems to be missing the X and S restriction sites. Digestion by these enzymes fails
Agarose electrophoresis of part K206009 as ordered from the registry in spring 2011 digested with several iGEM enzymes. nd stands for "non digested" As shown above, the digestion by P linearizes the plasmid as expected but X and S have no effect compared to the non digested plasmid. Moreover, double digestion by X+P only linearizes the plasmid.
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