Difference between revisions of "Part:BBa K902058"

Revision as of 23:59, 26 October 2012

hpaC inducible generator with lacI promotor (J04500)

This composite part contains the gene encoding E. coli's hpaC enzyme under control of the lacI promotor (BBa_J04500). HpaC is an oxidoreductase capable of recycling FMN to FMNH₂ while converting NADH to NAD. This part is used in conjuctions with the 4S desulfurization pathway to replace DszD. Doing so improves the rate of desulfurization 7-10x.

File-Ucalgary team sulfur 4s enzyme pathway diagram.png

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal SapI.rc site found at 509

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 <html><p>
-This composite part contains the gene encoding <I>E. coli's</i><a href="https://parts.igem.org/Part:BBa_K902057"></a><i>hpaC</i> enzyme under control of the lacI promotor (<a href="https://parts.igem.org/Part:BBa_J04500">BBa_J04500</a>).  HpaC is an oxidoreductase capable of recycling FMN to FMNH<sub>2</sub> while converting NADH to NAD. This part is used in conjuctions with the 4S desulfurization pathway to replace DszD. Doing so improves the rate of desulfurization 7-10x.</p><br>
+This composite part contains the gene encoding <I>E. coli's</i> <a href="https://parts.igem.org/Part:BBa_K902057"></a><i>hpaC</i> enzyme under control of the lacI promotor (<a href="https://parts.igem.org/Part:BBa_J04500">BBa_J04500</a>).  HpaC is an oxidoreductase capable of recycling FMN to FMNH<sub>2</sub> while converting NADH to NAD. This part is used in conjuctions with the 4S desulfurization pathway to replace DszD. Doing so improves the rate of desulfurization 7-10x.</p><br>
 </html>[[Image:File-Ucalgary team sulfur 4s enzyme pathway diagram.png|centre]]<html></html>