Difference between revisions of "Part:BBa K902004:Design"
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===Source=== | ===Source=== | ||
| − | This gene was amplified from the genome of E. coli via polymerase chain reaction. | + | This gene was amplified from the genome of <i>E. coli</i> via polymerase chain reaction. |
===References=== | ===References=== | ||
Yang M, Luoh SM, Goddard A, Reilly D, Henzel W, Bass S. The bglX gene located at 47.8 min on the Escherichia coli chromosome encodes a periplasmic beta-glucosidase.Microbiology. 1996 Jul;142 ( Pt 7):1659-65. | Yang M, Luoh SM, Goddard A, Reilly D, Henzel W, Bass S. The bglX gene located at 47.8 min on the Escherichia coli chromosome encodes a periplasmic beta-glucosidase.Microbiology. 1996 Jul;142 ( Pt 7):1659-65. | ||
Latest revision as of 02:37, 4 October 2012
Periplasmic beta-D-glucoside glucohydrolase (bglX)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 1621
Illegal AgeI site found at 1843
Illegal AgeI site found at 2032 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
An illegal pstI site at the 15th and 16th amino acid position was mutated out via site-directed mutagenesis.
Source
This gene was amplified from the genome of E. coli via polymerase chain reaction.
References
Yang M, Luoh SM, Goddard A, Reilly D, Henzel W, Bass S. The bglX gene located at 47.8 min on the Escherichia coli chromosome encodes a periplasmic beta-glucosidase.Microbiology. 1996 Jul;142 ( Pt 7):1659-65.