Difference between revisions of "Part:BBa K902018:Design"
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===Design Notes=== | ===Design Notes=== | ||
− | + | <html>This system must be grown on LB containing 10mM+ concentration of MgCl<sub>2</sub> because the system is active at low concentrations of magnesium and will kill the cells, or select for escapers, i.e, mutate out the regulatory elements.</html> | |
===Source=== | ===Source=== | ||
− | MgtAp, mgtArb, and S7 were synthesized | + | MgtAp, mgtArb, and S7 were synthesized. Then these parts were constructed together using the Biobrick assembly method. |
− | + | ||
===References=== | ===References=== | ||
Michael J. Cromie and Eduardo A. Groisman. Promoter and Riboswitch Control of the Mg2+ Transporter MgtA from Salmonella enterica. J Bacteriol. 2010 January; 192(2): 604–607. | Michael J. Cromie and Eduardo A. Groisman. Promoter and Riboswitch Control of the Mg2+ Transporter MgtA from Salmonella enterica. J Bacteriol. 2010 January; 192(2): 604–607. |
Latest revision as of 02:14, 4 October 2012
MgtAp-mgtArb-S7
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
This system must be grown on LB containing 10mM+ concentration of MgCl2 because the system is active at low concentrations of magnesium and will kill the cells, or select for escapers, i.e, mutate out the regulatory elements.
Source
MgtAp, mgtArb, and S7 were synthesized. Then these parts were constructed together using the Biobrick assembly method.
References
Michael J. Cromie and Eduardo A. Groisman. Promoter and Riboswitch Control of the Mg2+ Transporter MgtA from Salmonella enterica. J Bacteriol. 2010 January; 192(2): 604–607.