Difference between revisions of "Part:BBa K850001:Experience"

Revision as of 03:30, 4 October 2012

BBa_K850001 was excised from the Biobrick vector plasmid using Xba1 and Spe1 and cloned into the expression vector pUC19. Cell lysates from transformed and untransformed cells were compared by electrophoresis on a denaturing 12% polyacrylamide gel. Cells containing the BBa_K850001 insert in the proper orientation expressed a protein of about 26kD. The predicted molecular weight of NAG1 is 27.5 kD, strongly suggesting that the correct protein is being expressed.

Applications of BBa_K850001

User Reviews

UNIQb2c30b4e3b20094a-partinfo-00000000-QINU UNIQb2c30b4e3b20094a-partinfo-00000001-QINU

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 __NOTOC__
-This experience page is provided so that any user may enter their experience using this part.<BR>Please enter
+BBa_K850001 was excised from the Biobrick vector plasmid using Xba1 and Spe1 and cloned into the expression vector pUC19. Cell lysates from transformed and untransformed cells were compared by electrophoresis on a denaturing 12% polyacrylamide gel. Cells containing the BBa_K850001 insert in the proper orientation expressed a protein of about 26kD. The predicted molecular weight of NAG1 is 27.5 kD, strongly suggesting that the correct protein is being expressed.
-how you used this part and how it worked out.
 [[Image:Nag1.jpg]]