Difference between revisions of "Part:BBa K900001:Design"

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===Design Notes===
 
===Design Notes===
 
Codon optimization for yeast and internal restriction site removal to be compatible with our Golden Gate cloning scheme.  
 
Codon optimization for yeast and internal restriction site removal to be compatible with our Golden Gate cloning scheme.  
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===Source===
 
===Source===

Revision as of 01:40, 4 October 2012

PAGFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Codon optimization for yeast and internal restriction site removal to be compatible with our Golden Gate cloning scheme.

Source

Synthesized in house and cloned into Addgene plasmid [http://www.addgene.org/11911/ l11911] Previously engineered version of RFP from Patterson and Lippincott-Schwartz, 2002.

References

Patterson, G. H. & Lippincott-Schwartz, J. A Photoactivatable GFP for Selective Photolabeling of Proteins and Cells. Science 297, 1873–1877 (2002).