Difference between revisions of "Part:BBa K842005"
 
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Cloning and Uses:
 
Cloning and Uses:
 
This part was generated via PCR from the E. coli strain DH5α and cloned into the vector pSB1C3.  It is to be used in conjunction with the entire flagella synthesis genetic pathway to reproduce a complete flagella apparatus.
 
This part was generated via PCR from the E. coli strain DH5α and cloned into the vector pSB1C3.  It is to be used in conjunction with the entire flagella synthesis genetic pathway to reproduce a complete flagella apparatus.
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<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here
 
===Usage and Biology===
 
===Usage and Biology===

Latest revision as of 01:09, 4 October 2012

fliH

USC flih.gif

FliH functions as part of the flagella specific export system and is necessary for flageller growth and assembly. It is believed to be part of a two-component system consisting of fliH and fliL that hydrolyses ATP until construction of the flagella apparatus is completed.

Used for reconstitution of flagella apparatus in E. coli B strains.

Cloning and Uses: This part was generated via PCR from the E. coli strain DH5α and cloned into the vector pSB1C3. It is to be used in conjunction with the entire flagella synthesis genetic pathway to reproduce a complete flagella apparatus.














Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]