Difference between revisions of "Part:BBa K892009"

Revision as of 23:38, 3 October 2012

fucO

The gene fucO, which codes for glycolaldehyde reductase, is one of the genes required for E. coli to utilize ethylene glycol as a food source. The coding sequence is put behind a strong biofab promoter and RBS. This part should be used with aldA for the desired effect.

Usage and Biology

This part was characterized by [http://2012.igem.org/Team:Washington Washington 2012] using the turbidostat. MG1655 cells were cotransformed with fucO and aldA, the assay is described on the [http://2012.igem.org/Team:Washington/Protocols/EG_Assay wiki] and some of the data is shown below.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal PstI site found at 251
12
INCOMPATIBLE WITH RFC[12]
Illegal PstI site found at 251
21
COMPATIBLE WITH RFC[21]
23
INCOMPATIBLE WITH RFC[23]
Illegal PstI site found at 251
25
INCOMPATIBLE WITH RFC[25]
Illegal PstI site found at 251
Illegal AgeI site found at 967
Illegal AgeI site found at 1168
1000
COMPATIBLE WITH RFC[1000]

@@ Line 7: / Line 7: @@
 This part was characterized by [http://2012.igem.org/Team:Washington Washington 2012] using the turbidostat. MG1655 cells were cotransformed with fucO and aldA, the assay is described on the [http://2012.igem.org/Team:Washington/Protocols/EG_Assay wiki] and some of the data is shown below.
-[[Image:Washington_Turbidostat_Pump.png|border|400px|left|thumb|M9 30 mM ethylene glycol media injected into the culture vessel every minute to maintain the arbitrary optical density of 0.3.]]
-[[Image:Washington_Turbidostat_OD.png|border|400px|left|thumb|Optical density measurements of <i>E. coli</i> transformed with fucO pGA3K3 + aldA pGA1C3 within the turbidostat growing on M9 30 mM ethylene glycol media.]]
-I will add some words here.
 <!-- Add more about the biology of this part here
 ===Usage and Biology===