Difference between revisions of "Part:BBa K801000:Design"

(Design Notes)
(Source)
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'''Source:'''<br>
 
'''Source:'''<br>
<!--*Commercial system: plasmid name, system name, company name-->
+
Commercial system: pYES2 , Invitrogen
 
<!--*Plasmid: p???, provided by ?name_of_person?, ?institute/university?, ?country?-->
 
<!--*Plasmid: p???, provided by ?name_of_person?, ?institute/university?, ?country?-->
 
<!--*Preexisting BioBrick ?Bba_number?-->
 
<!--*Preexisting BioBrick ?Bba_number?-->
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<!--Thank you very much for using BBF RFC 82 to describe the present BioBrick. If you have any remarks or recommendations concerning this RFC we would highly appreciate to get your feedback on: []-->
 
<!--Thank you very much for using BBF RFC 82 to describe the present BioBrick. If you have any remarks or recommendations concerning this RFC we would highly appreciate to get your feedback on: []-->
 
  
 
===References===
 
===References===

Revision as of 08:23, 15 October 2012

pTUM100 yeast shuttle vector based on pYES2


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found at 36
    Illegal suffix found at 114
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 36
    Illegal NheI site found at 3379
    Illegal SpeI site found at 115
    Illegal PstI site found at 129
    Illegal NotI site found at 42
    Illegal NotI site found at 122
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 36
    Illegal XhoI site found at 63
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found at 36
    Illegal suffix found at 115
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found at 36
    Illegal suffix found at 105
    Illegal XbaI site found at 51
    Illegal NgoMIV site found at 69
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BsaI site found at 79
    Illegal BsaI.rc site found at 1536
    Illegal BsaI.rc site found at 2482
    Illegal SapI site found at 453
    Illegal SapI site found at 2634



Keywords:

yeast shuttle vector, yeast, yeast vector, pTUM, pYES2


Abbreviations:

Design Notes

Related BioBrick:

Other versions:

Quality control measures:

  • Part was totally sequenced

Backbone:

Protein coding:

Enzymatic activity:

Cytotoxicity:

Safety notes:

Intellectual property:

Corresponding part author/authors:

Source

Source:
Commercial system: pYES2 , Invitrogen


Organism:


References

Literature references:

Database references: