Difference between revisions of "Help:Reporters"
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[[Image:Part icon reporter.png]] | [[Image:Part icon reporter.png]] | ||
| − | <small>Browse | + | <small>Browse [https://parts.igem.org/cgi/partsdb/pgroup.cgi?pgroup=reporter reporter parts]!</small> |
<hr> | <hr> | ||
| + | ==Biology Background== | ||
All of our reporter genes encode for fluorescent proteins (FP's). These fluorescent proteins are derived from two different families of proteins: the Aeqoria Victoria jellyfish, which produces a wild-type GFP, and the Discosoma corals, which (after heavy modification) produces a monomeric RFP (mRFP).<br> | All of our reporter genes encode for fluorescent proteins (FP's). These fluorescent proteins are derived from two different families of proteins: the Aeqoria Victoria jellyfish, which produces a wild-type GFP, and the Discosoma corals, which (after heavy modification) produces a monomeric RFP (mRFP).<br> | ||
| − | + | ==Subtypes== | |
| + | The Registry's [https://parts.igem.org/cgi/partsdb/pgroup.cgi?pgroup=reporter reporter parts] are further subdivided into the following classifications based on their | ||
| + | ===Reporter Coding Regions=== | ||
| + | These are actually [[Basic Parts]] which code for reporter proteins. On their own, these parts are <b>NOT</b> capable of expressing these proteins. In order to express the reporter parts listed here, you will need an additional [[Help:Regulatory|regulatory]] region and a [[Help:Ribosome Binding Site|ribosome binding site]] preceeding this coding part. | ||
| + | |||
| + | ===Constituitive Reporters=== | ||
| + | These are full [[Composite Parts]] incorporating the Reporter Coding Region parts under constitutive [[Help:Regulatory|regulatory parts]]. These are devices which are capable of expressing proteins on their own. | ||
| + | <br> | ||
| + | <br> | ||
| + | ==Quantification== | ||
You can find estimates of excitation wavelengths for various fluorescent proteins [https://parts.igem.org/wiki/index.php/Fluorescent_proteins here]. <br> | You can find estimates of excitation wavelengths for various fluorescent proteins [https://parts.igem.org/wiki/index.php/Fluorescent_proteins here]. <br> | ||
| + | ==Links== | ||
For more on our fluorescent protein parts, see the [https://parts.igem.org/wiki/index.php/Fluorescent_proteins featured flourescent parts] | For more on our fluorescent protein parts, see the [https://parts.igem.org/wiki/index.php/Fluorescent_proteins featured flourescent parts] | ||
Revision as of 20:49, 25 June 2006
Browse reporter parts!
Contents
Biology Background
All of our reporter genes encode for fluorescent proteins (FP's). These fluorescent proteins are derived from two different families of proteins: the Aeqoria Victoria jellyfish, which produces a wild-type GFP, and the Discosoma corals, which (after heavy modification) produces a monomeric RFP (mRFP).
Subtypes
The Registry's reporter parts are further subdivided into the following classifications based on their
Reporter Coding Regions
These are actually Basic Parts which code for reporter proteins. On their own, these parts are NOT capable of expressing these proteins. In order to express the reporter parts listed here, you will need an additional regulatory region and a ribosome binding site preceeding this coding part.
Constituitive Reporters
These are full Composite Parts incorporating the Reporter Coding Region parts under constitutive regulatory parts. These are devices which are capable of expressing proteins on their own.
Quantification
You can find estimates of excitation wavelengths for various fluorescent proteins here.
Links
For more on our fluorescent protein parts, see the featured flourescent parts