Difference between revisions of "Part:BBa K801063:Design"
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<br>'''Keywords:''' | <br>'''Keywords:''' | ||
<!--These keywords are necessary to find your part using a fulltext sarch.--> | <!--These keywords are necessary to find your part using a fulltext sarch.--> | ||
− | + | limonen | |
<br>'''Abbreviations:''' | <br>'''Abbreviations:''' | ||
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'''Backbone:'''<br> | '''Backbone:'''<br> | ||
− | + | *Backbone name: pSB1C3' | |
<!--*Resistance: Amp/Cp/Kan/Tet--> | <!--*Resistance: Amp/Cp/Kan/Tet--> | ||
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'''Enzymatic activity:''' | '''Enzymatic activity:''' | ||
− | + | EC-number 4.2.3.20 | |
'''Cytotoxicity:'''<br> | '''Cytotoxicity:'''<br> | ||
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'''Organism:'''<br> | '''Organism:'''<br> | ||
− | + | *Genesequence derived from ''citrus limon'' | |
<!--*Codonoptimized for ''?organism_name?''--> | <!--*Codonoptimized for ''?organism_name?''--> | ||
<!--*Designed for the following Chassis: ''?organism-name?''--> | <!--*Designed for the following Chassis: ''?organism-name?''--> |
Revision as of 15:28, 3 October 2012
(+)-limonene synthase 1 expression cassette for yeast
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 2127
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 211
Illegal SapI.rc site found at 561
This is a composite part consisting of the yeast TEF1 promoter (BBa_K319003), the Citrus limon (+)-limonene synthase 1 coding region (BBa_K801061) and the yeast TEF1 terminator (BBa_K801011).
Keywords:
limonen
Abbreviations:
Design Notes
Related BioBrick:
- BBa_K801060: (+)-Limonene synthase 1 with Strep-tag and yeast consensus sequence
- BBa_K801061: (+)-Limonene synthase 1 coding region
- BBa_K801062: (+)-limonene synthase 1 expression cassette for yeast
- BBa_K801064: (+)-limonene synthase 1 expression cassette for yeast
- BBa_K801065: (+)-limonene synthase 1 with Strep-Tag
- BBa_K801066: (+)-limonene synthase 1 with Yeast Consensus Sequence for improved expression
Quality control measures:
Backbone:
- Backbone name: pSB1C3'
Protein coding:
Enzymatic activity: EC-number 4.2.3.20
Cytotoxicity:
Safety notes:
Intellectual property:
Corresponding part author/authors:
Source
Source:
Organism:
- Genesequence derived from citrus limon
References
Literature references:
Database references: