Difference between revisions of "Part:BBa K801096:Design"

(→‎Design Notes)
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'''Backbone:'''<br>
 
'''Backbone:'''<br>
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Backbone name: pSB1C3
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*Backbone name: pSB1C3
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Resistance: Cp
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*Resistance: Cp
−
<!--*Copynumber: low/medium/high-->
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*Copynumber: high
  
 
'''Protein coding:'''<br>
 
'''Protein coding:'''<br>

Revision as of 11:21, 3 October 2012

Aromatic prenyltransferase (APT) coding region


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Synthesized by Geneart, yeast codon optimized

http://www.ncbi.nlm.nih.gov/nuccore/AB543053


Keywords: Coumaryol-CoA, Coumaroyl-Coenzyme A, Xanthohumol, Biosynthesis, naringenin-chalcone synthase, O-methyltransferase 1


Abbreviations: APT = aromatic prenyltransferase

Design Notes

Related BioBrick:

Related BioBricks: BBa_K801090, BBa_K801091, BBa_K801092, BBa_K801093, BBa_K801094, BBa_K801095, BBa_K801097, BBa_K801098

Cloning details:
Designed in RFC10/RFC25

Quality control measures:
Part was totally sequenced

Backbone:

  • Backbone name: pSB1C3
  • Resistance: Cp
  • Copynumber: high

Protein coding:

  • Protein: aromatic prenyltransferase
  • Tag: c-terminally fused Strep

Enzymatic activity:
EC-number: 2.5.1

Cytotoxicity:

Safety notes:

Intellectual property:

Corresponding part author/authors:

Source

Source:
Synthesized by Geneart


Organism:
Genesequence derived from Humulus lupulus

Codonoptimized for Saccharomyces cerevisiae

References

Literature references:


Database references:
[http://www.ncbi.nlm.nih.gov/nuccore/AB543053.1 GenBank: Humulus lupulus mRNA for aromatic prenyltransferase, complete cds]

[http://www.uniprot.org/uniprot/E5RP65 Uniprot: Aromatic prenyltransferase]