Difference between revisions of "Part:BBa K902067:Design"

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===Design Notes===
 
===Design Notes===
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This construct was designed as part of a kill switch for 2012 iGEM Calgary project. Please view our [http://2012.igem.org/Team:Calgary/Project/HumanPractices/Killswitch Wiki] for more details about our kill system.
+
This construct was designed as part of a kill switch for 2012 iGEM Calgary project. Please view our [http://2012.igem.org/Team:Calgary/Project/HumanPractices/Killswitch Wiki] for more details about our kill system. This circuit needs to be grown up on special LB plates containing >0.2% glucose to repress this system successfully.
  
 
===Source===
 
===Source===

Revision as of 02:40, 4 October 2012

Rhamnose promoter with S7 micrococal nuclease


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This construct was designed as part of a kill switch for 2012 iGEM Calgary project. Please view our [http://2012.igem.org/Team:Calgary/Project/HumanPractices/Killswitch Wiki] for more details about our kill system. This circuit needs to be grown up on special LB plates containing >0.2% glucose to repress this system successfully.

Source

The pRha promoter and S7 nuclease were commercially synthesized. The RBS was taken from the Parts Registry.

References