Difference between revisions of "Part:BBa K902034:Design"
 
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===References===
 
===References===
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Shintani,M., Yoshida,T., Habe,H., Omori,T. and Nojiri,H. Large plasmid pCAR2 and class II transposon Tn4676 are functional mobile genetic elements to distribute the carbazole/dioxin-degradative car gene cluster in different bacteria. Appl. Microbiol. Biotechnol. 67 (3), 370-382 (2005).

Latest revision as of 01:58, 4 October 2012

CarAd Coding Sequence


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 63
    Illegal SapI.rc site found at 51


Design Notes

Site directed mutagenesis was required to remove a NotI site. Nucleotide 657 was changed from a G in the native DNA to an A in our construct. This was a silent mutation which had no effect on the amino acid output.


Source

Gene was amplified by PCR using Pseudomonas putida plasmid pCAR1.2 as a template

References

Shintani,M., Yoshida,T., Habe,H., Omori,T. and Nojiri,H. Large plasmid pCAR2 and class II transposon Tn4676 are functional mobile genetic elements to distribute the carbazole/dioxin-degradative car gene cluster in different bacteria. Appl. Microbiol. Biotechnol. 67 (3), 370-382 (2005).