Difference between revisions of "Part:BBa K902048:Experience"

Revision as of 08:47, 3 October 2012

XylE (J33204) was biobricked under the tetR promoter (R0040). This construct was confirmed by sequencing and used in visual assays. The visual assay shown below demonstrated that the XylE gene was expressed with the tetR promoter and that the resulting enzyme (catechol 2,3-dioxygenase) was functional. The colour change to bright yellow indicated the conversion of catechol to 2-hydroxymuconic semialdehyde.

Applications of BBa_K902048

User Reviews

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 __NOTOC__
-This experience page is provided so that any user may enter their experience using this part.<BR>Please enter
+XylE (J33204) was biobricked under the tetR promoter (R0040).  This construct was confirmed by sequencing and used in visual assays.  The visual assay shown below demonstrated that the XylE gene was expressed with the tetR promoter and that the resulting enzyme (catechol 2,3-dioxygenase) was functional.  The colour change to bright yellow indicated the conversion of catechol to 2-hydroxymuconic semialdehyde.
-how you used this part and how it worked out.
 ===Applications of BBa_K902048===